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Department of Biosciences

Brightfield & Widefield Fluorescence Microscopy

Brightfield and widefield fluorescence microscopes offer the simplest and most widely used methods in light microscopy.

Leica DMI 3000 + Microinjection

The Leica DMI3000 B DIC is linked up to an Eppendorf TransferMan NK2 and either the FemtoJet or FemtoJet Express. 

Student using Leica Microscope

 

General Specification

  • Inverted microscope
  • DIC (Differential Contrast)

 

  • Image acquisition or video capture via Panasonic 3CCD 1080p HD colour camera

Objectives Lenses

  • 5x N-PLAN - NA 0.12
  • 10x N-PLAN Ph - NA 0.25
  • 40x N-PLAN L Corr Ph2 - NA

Eppendorf TransferMan NK2 and either the FemtoJet or FemtoJet Express

The TransferMan NK2 allows the user to micro manipulate various samples, utilising the FemtoJet for injecting small volumes up to 100pL or the FemtoJet Express for volumes greater than 100pL. This system is also available on the upright DM2500. The injectors have variable injection pressure, timing and compensation pressure and are fully programmable which ensures reproducibility.