Lightsheet Microscopy
Lightsheet microscopy, otherwise know as Selective Plane Illumination Microscopy (SPIM), utilises a focussed sheet of light to illuminate a specimen. Lightsheet technology is a much gentler illumination technique, resulting in less-phototoxic imaging of samples and is very quick utilising cameras to capture images at high speed.
Lightsheet Microscope Systems
Zeiss Lattice Lightsheet 7
Durham's Bioimaging facility was awarded a BBSRC ALERT22 grant to purchase a dual camera Lattice Lightsheet microscope. In July 2024, the transformative system Zeiss LLS 7 was installed in the facility.
Thank you BBSRC!
General Specification
Inverted microscope
Environmental control - temperature, humidity & CO2
Objective effective magnification ~40x
Objective Lenses
Magnification varies with the meniscus, but is approximately 40 x
Excitation Laser Lines
488nm
561nm
640nm