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Lightsheet Microscopy

Lightsheet microscopy, otherwise know as Selective Plane Illumination Microscopy (SPIM), utilises a focussed sheet of light to illuminate a specimen. Lightsheet technology is a much gentler illumination technique, resulting in less-phototoxic imaging of samples and is very quick utilising cameras to capture images at high speed.

 

 

Zeiss Lattice Lightsheet 7

Durham's Bioimaging facility was awarded a BBSRC ALERT22 grant to purchase a dual camera Lattice Lightsheet microscope. In July 2024, the transformative system Zeiss LLS 7 was installed in the facility.

Thank you BBSRC!

Post doctoral student sitting by lattice lightsheet microscope and computer to control it

 

General Specification

Inverted microscope

Environmental control - temperature, humidity & CO2

Objective effective magnification ~40x

Objective Lenses

Magnification varies with the meniscus, but is approximately 40 x

Configuration of lens on the Zeiss Lattice Lightsheet, showing an effective magnification of 40x from the illumination objective and the detection optics.

Excitation Laser Lines

488nm

561nm

640nm