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Brightfield & Widefield Fluorescence Microscopy

Brightfield and widefield fluorescence microscopes offer the simplest and most widely used methods in light microscopy.

Zeiss Cell Observer

The Zeiss Cell Observer is a widefield fluorescence microscope system which allows you to visualize living organisms and intracellular processes, even over a period of several days, involving a number of different fluorescence channels, z-planes and positions. For long term imaging of cells the microscope is housed within an environmental chamber and has heated stage inserts providing precise control of temperature, CO2 and humidity. The objective lenses have been specially designed for live cell Imaging, including correction rings to avoid spherical aberration when imaging between 23-37C. This system can image multiple types of culture dishes including 6 well plates with the motorised stage allowing image capture from multiple points in each well in turn.

Microscope for imaging living cells

General Specification

Inverted microscope

Widefield fluorescence 

Environmental control - temperature, humidity & CO2

Filter Cube Specification

Ex 335-383nm/ Em 420-470nm     DAPI

Ex 425-448nm / Em 460-500nm    HE CFP

Ex 450-490nm / Em 500-550nm     Alexa Fluor 488

Ex 540-552nm/ Em 590LP               Alexa Fluor 546

Objective Lenses

  • 10 x - 0.3 NA
  • 20 x - 0.4 NA
  • 40 x - 0.6 NA